We are interested in how herpesviruses manipulate the host cell environment and use these viruses as a tool to discover new regulatory mechanisms in cell biology and immunology. A particular focus is on cytomegalovirus and herpes simplex virus 1 infection. We employ a broad range of systems biology methodology and integrative bioinformatic analyses to study virus-host interactions. We previously developed metabolic RNA labeling approaches (4sU-tagging) to increase the temporal resolution of gene expression profiling. We now pioneered metabolic RNA labeling using 4sU for single cell sequencing (single cell thiol-(SH)-linked) nucleotide conversion sequencing, sc2RNA-seq). We collaborate with various other groups to apply sc2RNA-seq to other model systems and develop new bioinformatics tools for integrative data analysis.