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https://www.singlecell.de/
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Virtual

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Next Event

Date

Mar 25 2022
Expired!

Time

CET
9:30 am - 10:15 am

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SCOG event

SCOG Virtual Lecture Series
‘Recent Advances in Single Cell Omics’:
35. Bart Deplancke (EPFL Lausanne)

We are excited to announce the next speaker in our SCOG Virtual Lecture Series ‘Recent Advances in Single Cell Omics’: Bart Deplancke (EPFL Lausanne)

 

Abstract:

‘Engineering next-generation single cell phenomics technologies’
Single cell transcriptomics (scRNA-seq) is transforming biomedical research by providing unique insights into a cell’s identity and characteristics. However, several key single cell-related technological challenges remain, including the need for methods that i) are tailored toward low-input cell samples to process small, individual tissues; ii) enable linking the transcriptome of a cell to a high-resolution image of that same cell; and 3) allow retrieving the transcriptome of a cell without lysing it to support downstream phenotyping or direct trajectory inference. In this technology-centric talk, I will present my lab’s efforts to address each of these challenges. Specifically, I will introduce DisCo, a deterministic, mRNA-capture bead and cell co-encapsulation dropleting system. DisCo enables precise particle and cell positioning and droplet sorting control through combined machine-vision and multilayer microfluidics, enabling continuous processing of low-input single cell suspensions at high capture efficiency. I thereby will discuss how further integration of high-resolution imaging into DisCo is paving the way for an interconnecting robotic image and scRNA-seq (IRIS) platform uniquely enabling the selection, sorting, and molecular as well as phenotypic characterization of individual cells. Finally, I will introduce Live-seq, a novel cell lysis-devoid, single cell transcriptomics approach that can act as a transcriptomic recorder to predict cell response heterogeneity and that can can be used to perform sequential transcriptome profiling on cells to directly map cell trajectories.

Biography:

Bart Deplancke graduated as a Biochemical Engineer at Ghent University (Belgium, 1998) after which he pursued doctoral studies in Immunobiology at the University of Illinois (USA) and postdoctoral work in regulatory genomics at the Harvard and UMass Medical Schools. In 2007, he established his lab at the EPFL School of Life Sciences where he is now Full Professor, Vice-Dean of Innovation, and Swiss Institute of Bioinformatics member and is developing new experimental and computational approaches to study the biology of the genome. The Deplancke Lab has by now built a sizeable toolkit, involving microfluidics, high-throughput sequencing, and single cell genomics. This toolkit is being used to address questions pertaining to cellular development, with a primary focus on adipose biology, as well as to how genomic variation affects molecular and organismal diversity. In 2017, he was elected to the National Research Council of the Swiss National Science Foundation, and in 2021, he received the 2021 Cloëtta Prize for outstanding contributions to biomedical research. He is also the co-founder of two Biotech companies (Genohm, Acquired by Agilent in 2018; and Alithea Genomics).

Further information on participation, the program and the application for a lecture can be found below.
  • Further Information:

     

    VIRTUAL LECTURE SERIES: 'Recent Advances in Single Cell Omics'
    • Biweekly event on Fridays at 9:30 am (CEST)
    • 30 min presentation, followed by 15 min discussion / Q&A
    SYNOPSIS

    The virtual lecture series includes biweekly presentations about current single cell research. In addition to selected presentations by invited guests, we would particularly like to offer SCOG partners a platform to present their work to the network (read below how to apply).
    The program will be updated regularly (see schedule below).

    PARTICIPATION

    The Virtual Lecture Series takes place via Zoom. Please register in order to receive information on how to join the webinar. Your personal join link will be valid for all upcoming lectures of the series.
    REGISTER HERE

    APPLY FOR LECTURE

    We would kindly like to encourage the SCOG community not only to participate as an attendee but to present their work to the community. All contributions are welcome.
    If you are a SCOG partner (PI/group leader) or member (Postdoc) and would like to give a talk during the lecture series, please apply by completing the form on our website. Please note that a balanced representation of topics, institutes and gender will be ensured in the selection of applications.

    APPLY HERE

Hourly Schedule

11 February 2022

9:30 - 10:15
Andreas Moor (ETH Zurich)
Spatial biology in intestinal homeostasis and cancer

11 March 2022

9:30 - 10:15
Kikue Tachibana (MPIB Munich)
Insights into the emergence of 3D genome architecture at the start of life by single-nucleus Hi-C

25 March 2022

9:30 - 10:15
Bart Deplancke (EPFL Lausanne)
Engineering next-generation single cell phenomics technologies

08 April 2022

9:30 - 10:15
Christoph Bock (CeMM Vienna)