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https://www.singlecell.de/
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Date

Sep 10 2021

Time

CEST
9:30 am - 10:15 am

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SCOG event

SCOG Virtual Lecture Series
‘Recent Advances in Single Cell Omics’:
26. Peter Tessarz (MPI Age, Cologne)

We are excited to announce the next speaker in our SCOG Virtual Lecture Series ‘Recent Advances in Single Cell Omics’:  Peter Tessarz (MPI Age, Cologne)
  • Title: ‘Single cell epigenomics reveal zonation-specific differences in the ageing of liver and establish organoids as an ex vivo model system for ageing research’
Ageing is accompanied by a gradual decline of physiological function. Tissue-wide studies have revealed that the epigenome and transcriptome change during the lifetime of an organism – sometimes precise enough to calculate biological and chronological age based on the DNA methylation and/or the transcriptome. This age-dependent difference observed in bulk data is rarely visible in single cell RNA-seq data. We wondered if it was possible to capture ageing at single cell resolution by investigating the epigenome as a proxy for an underlying control level. We used liver as a model and scATAC-seq, which enabled us to identify all major cell types of the tissue. Interestingly, we did not observe a major difference in the ageing epigenome for the majority of liver resident cell types. However, hepatocytes showed a very clear separation based on age. Intriguingly, this separation was not only age-dependent, but showed a clear connection to the hepatocyte location within the liver lobule, thus on liver zonation. Liver zonation is a functional hallmark of the liver and describes the separation of labor within the liver lobule. Phenotypically, zonation is also visible in old livers as the majority of fat deposits occurs around the central vein. Using spatial transcriptomics, we confirmed on the molecular level the zonal differences on old livers and identified marker genes. Furthermore, integration of scATAC-seq with publicly available scRNA-seq data allowed us to address how chromatin architecture contributes to the increase in transcriptional noise with age.
Adult progenitor cells diminish in the liver tissue during ageing. In order to enrich for this rare progenitor population, we established liver-derived organoids. Using scATAC and scRNA-seq, we can show that under the growth conditions used, we generated organoids that show markers of progenitors, but also of hepatocytes. This is also visible on a functional level. Intriguingly, the organoids possess an epigenetic memory of their tissue of origin. Pathways controlling fatty acid and drug metabolism remain altered in the organoid on an epigenetic, transcriptional and functional level making them a potential ex vivo model for liver ageing.
Further information on participation, the program and the application for a lecture can be found below.

VIRTUAL LECTURE SERIES: ‘Recent Advances in Single Cell Omics’
  • Biweekly event on Fridays at 9:30 am (CEST)
  • 30 min presentation, followed by 15 min discussion / Q&A
SYNOPSIS
The virtual lecture series includes biweekly presentations about current single cell research. In addition to selected presentations by invited guests, we would particularly like to offer SCOG partners a platform to present their work to the network (read below how to apply).
The program will be updated regularly (see schedule below).
PARTICIPATION
The Virtual Lecture Series takes place via Zoom. Please register in order to receive information on how to join the webinar. Your personal join link will be valid for all upcoming lectures of the series. REGISTER HERE.
Please note: We are changing the platform from GoToWebinar to Zoom! You have to register for the lecture series again, even if you have already registered before.  
APPLY FOR LECTURE
We would kindly like to encourage the SCOG community not only to participate as an attendee but to present their work to the community. All contributions are welcome.
If you are a SCOG partner (PI/group leader) or member (Postdoc) and would like to give a talk during the lecture series, please APPLY BY COMPLETING THIS FORM.
Please note that a balanced representation of topics, institutes and gender will be ensured in the selection of applications.

Hourly Schedule

Friday 15 January 2021

9:30am - 10:15am
Decoding host-pathogen interactions one cell at the time
Emmanuel Saliba (HZI/HIRI Würzburg)

Friday 29 January 2021

9:30am - 10:15am
Human organoid development through the lens of single-cell genomics
Barbara Treutlein (ETH Zurich)

Friday 26 February 2021

9:30am - 10:15am
Single cell computational epigenomics
Maria Colomé-Tatché (HMGU Munich)

Friday 12 March 2021

9:30am - 10:15am
Roles of Polycomb in Embryogenesis: Insights from single-cell transcriptomics
Stefanie Grosswendt (BIMSB/MDC Berlin)

Friday 26 March 2021

9:30am - 10:15am
Chromatin and gene-regulatory dynamics of the developing human cerebral cortex at single-cell resolution
Fabian Müller (USAAR Saarbrücken)

Friday 09 April 2021

4pm - 5:15pm
tbd
Samantha Morris (WUSTL, St. Louis, USA)

Friday 23 April 2021

9:30am - 10:15am
scRNA-seq reveals a macrophage subset that provides a splenic replication niche for intracellular Salmonella
Roi Avraham (Weizmann Institute of Science, Israel)

Friday 7 May 2021

9:30am - 10:15am
tbd
Roser Vento-Tormo (Wellcome Sanger Institute, UK)

Friday 21 May 2021

9:30am - 10:15am
tbd
Emanuel Wyler (BIMSB/MDC, Berlin)

Friday 4 June 2021

9:30am - 10:15am
tbd
Herbert Schiller (HMGU, Munich)

Friday 2 July 2021

9:30am - 10:15am
tbd
Simon Haas (BIH@Charité, BIMSB/MDC, Berlin)

Friday 16 July 2021

9:30am - 10:15am
tbd
tbc

Friday 10 September 2021

9:30am - 10:15am
Single cell epigenomics reveal zonation-specific differences in the ageing of liver and establish organoids as an ex vivo model system for ageing research
Peter Tessarz (MPI Age, Cologne)

Friday 24 September 2021

9:30am - 10:15am
tbd
tbc

Friday 08 October 2021

9:30am - 10:15am
tbd
Heiko Lickert (HMGU, Munich)

Friday 22 October 2021

9:30am - 10:15am
tbd
Michela Deleidi (DZNE, Tübingen)

Friday 05 November 2021

9:30am - 10:15am
How proliferation cell cycle dynamics affect the responses of single cells to chemotherapy
Adrian Granada (Charité, Berlin)