SCOG Virtual Lecture Series
‘Recent Advances in Single Cell Omics’:
19. Guest: Samantha Morris (WUSTL, St. Louis, USA)

We are excited to announce the next speaker in our SCOG Virtual Lecture Series ‘Recent Advances in Single Cell Omics’:  Samantha Morris (WUSTL, St. Louis, USA)

• Title: ‘New single-cell technologies to dissect reprogramming and development’

Direct lineage reprogramming involves the remarkable conversion of cellular identity. Single-cell technologies aid in deconstructing the considerable heterogeneity in transcriptional states that typically arise during lineage conversion. However, lineage relationships are lost during cell processing, limiting accurate trajectory reconstruction. We previously developed ‘CellTagging’, a combinatorial cell indexing methodology, permitting the parallel capture of clonal history and cell identity, where sequential rounds of cell labeling enable the construction of multi-level lineage trees. CellTagging and longitudinal tracking of fibroblast to induced endoderm progenitor (iEP) reprogramming reveals two distinct trajectories: one leading to successfully reprogrammed cells, and one leading to a dead-end state. Here, I present two new methods to enable the molecular mechanisms underlying reprogramming outcome to be dissected. The first is an experimental method, ‘Calling Cards’, enabling transcription factor binding to be recorded, in individual cells, in the earliest stages of reprogramming. The second method is a new computational platform, called ‘CellOracle’, that uses single-cell transcriptome and chromatin accessibly data to reconstruct changes in GRN configurations across the reprogramming process. Together, these tools provide new mechanistic insights into how transcription factors can drive changes in cell identity, and help reveal new factors to enhance the efficiency and fidelity of reprogramming.

Further information on participation, the program and the application for a lecture can be found below.

VIRTUAL LECTURE SERIES: ‘Recent Advances in Single Cell Omics’

• Biweekly event on Fridays at 9:30 am (CEST)

• 30 min presentation, followed by 15 min discussion / Q&A

SYNOPSIS

The virtual lecture series includes biweekly presentations about current single cell research. In addition to selected presentations by invited guests, we would particularly like to offer SCOG partners a platform to present their work to the network (read below how to apply).

The program will be updated regularly (see schedule below).

PARTICIPATION

The Virtual Lecture Series takes place via Zoom. Please register in order to receive information on how to join the webinar. Your personal join link will be valid for all upcoming lectures of the series. REGISTER HERE.

Please note: We are changing the platform from GoToWebinar to Zoom! You have to register for the lecture series again, even if you have already registered before.  

APPLY FOR LECTURE

We would kindly like to encourage the SCOG community not only to participate as an attendee but to present their work to the community. All contributions are welcome.

If you are a SCOG partner (PI/group leader) or member (Postdoc) and would like to give a talk during the lecture series, please APPLY BY COMPLETING THIS FORM.

Please note that a balanced representation of topics, institutes and gender will be ensured in the selection of applications.